| Product Information | |
| Name | Rat Immunoglobulin G2a (IgG2a) quantitative detection ELISA Kit |
| Item No. | EC014 |
| Principle | This ELISA kit applies the quantitative sandwich enzyme immunoassay technique. |
| Specificity | This kit identifies natural and recombinant Rat IgG2a |
| Storage | 6 months at 2-8°C |
| Sensitivity | 1 pg/ml |
| Description | This kit is used for detecting the IgG2a concentration of serum, plasma, and cell culture supernatant. |
| Intra-Assay | <15% |
| Inter-Assay | <12% |
| Sample | serum, plasma, and cell culture supernatant. |
|
Description
|
This ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Rat IgG2A antibody (solid phase antigen), standards or samples are added to the microtiter plate wells, then add anti- Rat IgG2A HRP labeled antibody. Wells are thoroughly washed to remove all unbound components. And a sandwich complex of solid antigen-antibody enzyme-labeled secondary antibodies is formed on the solid surface of the microplate. Next, Substrate solution A and substrate solution B are added to each well, and it will produce blue materials under HRP catalysis. The enzyme-substrate reaction is terminated by the addition of sulphuric acid and the color change to yellow. Measured spectrophotometrically at a wavelength of 450 nm, the absorbance (OD value) is positively correlated with the concentration of Rat IGG2A in the sample. The concentration of IgG2A in the samples could be determined by comparing the O.D. of the samples to the standard curve.
|
